Recombinant FOXA1 anticorps (AA 372-472)

N° du produit ABIN7654217

L’anticorps Lapin Monoclonal anti-FOXA1 a été validé pour WB, IHC, FACS, IF et IHC (fp). Il convient pour détecter FOXA1 dans des échantillons de Humain et Rat.

Aperçu rapide pour Recombinant FOXA1 anticorps (AA 372-472) (ABIN7654217)

Antigène: FOXA1 (Forkhead Box A1 (FOXA1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp FOXA1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: FOXA1-2230R

Détail du produit anti-FOXA1 anticorps

Épitope: AA 372-472

Fonction: FOXA1 / HNF3A (FOXA1/2230R)

Attributs du produit: The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1 (alpha and beta), HNF-3 (alpha, beta and gamma), HNF-4 (alpha and gamma), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human FOXA1 protein fragment (around aa372-472)

Isotype: IgG

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1.0 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: HepG-2 or MCF-7 cells. Liver, Pancreas, Bladder, Prostate, Colon and Lung and Breast.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur FOXA1

Antigène: FOXA1 (Forkhead Box A1 (FOXA1))

Autre désignation: FOXA1

Sujet:

Synonyms: Forkhead box protein A1 (FOXA1), Hepatocyte nuclear factor 3-alpha (HNF-3-alpha or HNF-3A or HNF3A), Transcription factor 3A (TCF-3A or TCF3A)

Gene Symbol: FOXA1

Poids moléculaire: 49 kDa

ID gène: 3169

UniProt: P55317

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis